Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: C9orf72 knockdown is essential to trigger poly(GP) toxicity, inducing paralysis due to motor neuron degeneration.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques: Knockdown

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Poly(GP) and Sqstm1/p62 accumulate preferentially in motor neurons under c9orf72 knockdown.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques: Knockdown

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Autophagy induction rescues the synergistic toxicity of C9orf72 gain and loss of function.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Proteomics indicates deficit in mitochondria function in motor neurons of C9orf72 gain and loss of function zebrafish.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Mitophagy is altered under the synergistic effects of C9orf72 gain and loss of function.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: C9orf72 pathology induces motor neurons apoptosis.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Proposed mechanisms for poly(GP) toxicity in C9orf72 haploinsufficiency. C9orf72 knockdown induces poly(GP) and SQSTM1/p62 accumulation in motor neurons due to autophagy alteration. These synergistic effects of C9orf72 loss of function and poly(GP) expression perturb mitochondrial homeostasis, including mitophagy deregulation. Subsequently, accumulated abnormal mitochondria engage the cell death cascade through cascade activation, finally resulting in motor neuron degeneration through apoptosis cell death and arising motor deficits. Several drugs have been identified as being able to counteract motor deficits in this C9orf72 model: the MTOR inhibitor rapamycin and the PIKFYVE inhibitor apilimod, both by activating autophagy; the mitophagy activator urolithin A; and decylubiquinone, an analogue of ubiquinone (coenzyme Q10) and inhibitor of mitochondrial depolarization.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques: Knockdown, Expressing, Activation Assay

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: C9orf72 knockdown is essential to trigger poly(GP) toxicity, inducing paralysis due to motor neuron degeneration.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques: Knockdown

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Poly(GP) and Sqstm1/p62 accumulate preferentially in motor neurons under c9orf72 knockdown.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques: Knockdown

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Autophagy induction rescues the synergistic toxicity of C9orf72 gain and loss of function.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Proteomics indicates deficit in mitochondria function in motor neurons of C9orf72 gain and loss of function zebrafish.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Mitophagy is altered under the synergistic effects of C9orf72 gain and loss of function.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: C9orf72 pathology induces motor neurons apoptosis.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques:

Journal: Autophagy

Article Title: Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

doi: 10.1080/15548627.2024.2358736

Figure Lengend Snippet: Proposed mechanisms for poly(GP) toxicity in C9orf72 haploinsufficiency. C9orf72 knockdown induces poly(GP) and SQSTM1/p62 accumulation in motor neurons due to autophagy alteration. These synergistic effects of C9orf72 loss of function and poly(GP) expression perturb mitochondrial homeostasis, including mitophagy deregulation. Subsequently, accumulated abnormal mitochondria engage the cell death cascade through cascade activation, finally resulting in motor neuron degeneration through apoptosis cell death and arising motor deficits. Several drugs have been identified as being able to counteract motor deficits in this C9orf72 model: the MTOR inhibitor rapamycin and the PIKFYVE inhibitor apilimod, both by activating autophagy; the mitophagy activator urolithin A; and decylubiquinone, an analogue of ubiquinone (coenzyme Q10) and inhibitor of mitochondrial depolarization.

Article Snippet: Samples were cut into 20-μm thick transversal sections which were blocked and permeabilized with 0.2% gelatin (Sigma, 04055), 0.25% Triton X-100 (Thermo Fisher Scientific, 85112) diluted in 1X PBS (Thermo Fisher Scientific, 11594516) and incubated overnight with primary antibodies: anti-GFP (1:500, chicken polyclonal; GeneTex, GTX13970), anti-SQSTM1 (1:250, mouse monoclonal; Santa Cruz Biotechnology, sc-28359), anti-TOMM20 (1:200, rabbit polyclonal; Sigma Aldrich, HPA011562), anti-cleaved CASP3 (1:250, rabbit polyclonal; Cell Signaling Technology, 9661), C9orf72 (1:50, mouse, provided by Dr Nicolas Charlet-Berguerand [IGBMC, Illkirch, France]), Mrpl12 (1:200, rabbit polyclonal; Proteintech, 14795), Mrpl44 (1:200, rabbit polyclonal; Proteintech, 16394), Mrps34 (1:100, mouse polyclonal; Sigma Aldrich, KPAO42112) and/or Mrpl37 (1:100, rabbit polyclonal; Sigma Aldrich, HPA025826).

Techniques: Knockdown, Expressing, Activation Assay

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.e17-12-0703

Figure Lengend Snippet: Figure 1. WDR41 interacts with C9orf72 but is not required for the stability of C9orf72

Article Snippet: GFP-tagged full length C9orf72 (residues 1-481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1-937, Uniprot identifier Q8TEV9-1) were described previously (Amick et al., 2016). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74159) (Sellier et al., 2016b).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.e17-12-0703

Figure Lengend Snippet: Figure 2. WDR41 is required for the recruitment of C9orf72 to lysosomes. (A)

Article Snippet: GFP-tagged full length C9orf72 (residues 1-481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1-937, Uniprot identifier Q8TEV9-1) were described previously (Amick et al., 2016). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74159) (Sellier et al., 2016b).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.e17-12-0703

Figure Lengend Snippet: Figure 3. WDR41 is required for C9orf72 and SMCR8 enrichment on purified lysosomes.

Article Snippet: GFP-tagged full length C9orf72 (residues 1-481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1-937, Uniprot identifier Q8TEV9-1) were described previously (Amick et al., 2016). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74159) (Sellier et al., 2016b).

Techniques: Purification

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.e17-12-0703

Figure Lengend Snippet: Figure 8. WDR41-dependent recruitment of C9orf72 to lysosomes is critical for the

Article Snippet: GFP-tagged full length C9orf72 (residues 1-481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1-937, Uniprot identifier Q8TEV9-1) were described previously (Amick et al., 2016). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74159) (Sellier et al., 2016b).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.e17-12-0703

Figure Lengend Snippet: Figure 3. WDR41 is required for C9orf72 and SMCR8 enrichment on purified lysosomes.

Article Snippet: GFP-tagged full length C9orf72 (residues 1-481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1-937, Uniprot identifier Q8TEV9-1) were described previously (Amick et al., 2016). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74159) (Sellier et al., 2016b).

Techniques: Purification

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.E17-12-0703

Figure Lengend Snippet: WDR41 interacts with C9orf72 but is not required for the stability of C9orf72 and SMCR8. (A) Immunoblot analysis of WDR41 KO in HEK293FT cells that express 2xHA-tagged C9orf72 from the endogenous locus. (B) Immunoblot analysis of control and WDR41 KO HeLa cells. (C) Endogenously expressed 2x-HA C9orf72 was immunoprecipitated from the parental and WDR41 knockout cell line, followed by immunoblotting for the indicated proteins. (D) Anti-HA immunoprecipitation of control and WDR41-HA-transfected HeLa cells followed by immunoblotting for the indicated proteins. (E) C9orf72/SMCR8 double knockout cells were transfected with GFP, GFP-C9orf72, or SMCR8-GFP followed by anti-GFP immunoprecipitation and immunoblotting for the indicated proteins. (F) Immunoprecipitation results in E support a model wherein WDR41 associates with the C9orf72:SMCR8 complex primarily via an interaction with C9orf72.

Article Snippet: GFP-tagged full-length C9orf72 (residues 1–481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1–937, Uniprot identifier Q8TEV9-1) were described previously ( Amick et al. , 2016 ). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand, Université de Strasbourg, Illkirch, France (Addgene plasmid #74159) ( Sellier et al. , 2016 ).

Techniques: Western Blot, Control, Immunoprecipitation, Knock-Out, Transfection, Double Knockout

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.E17-12-0703

Figure Lengend Snippet: WDR41 is required for the recruitment of C9orf72 to lysosomes. (A) Immunofluorescence images of C9orf72 localization (endogenously expressed 2xHA-C9orf72) in starved wild-type, WDR41 knockout, and WDR41 KO cells that were rescued by stable expression of a WDR41 transgene. Localization of C9orf72 to lysosomes (LAMP1 signal) is lost in WDR41 knockout cells. Scale bar, 10 μm. (B) For the indicated cell lines, the percentage of cells in starved conditions containing C9orf72 puncta that colocalize with LAMP1 are indicated. (C) Immunoblot analysis of WDR41 levels in wild-type, knockout, and rescue cell lines.

Article Snippet: GFP-tagged full-length C9orf72 (residues 1–481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1–937, Uniprot identifier Q8TEV9-1) were described previously ( Amick et al. , 2016 ). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand, Université de Strasbourg, Illkirch, France (Addgene plasmid #74159) ( Sellier et al. , 2016 ).

Techniques: Immunofluorescence, Knock-Out, Expressing, Western Blot

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.E17-12-0703

Figure Lengend Snippet: WDR41 is required for C9orf72 and SMCR8 enrichment on purified lysosomes. (A) Immunoblots of the indicated proteins in the total cell lysate (input) and in magnetically isolated lysosomes (lysosomes). Equal amounts of protein were loaded in each lane. LAMP1, Rab7a, Cathepsin D (mature form), and LAMTOR1 were used as markers of late endosomes/lysosomes; EEA, early endosomes; tubulin, actin, cytoskeleton; Lamin A/C, nucleus; Calnexin, ER; GM130, –Golgi. (B) Quantification of immunoblots for the indicated proteins expressed as a fold increase in the lysosome fraction relative to the input (mean ± SEM, n = 3). (C) Immunoblots of C9orf72, SMCR8, and LAMP1 in the total cell lysate (input) and in magnetically isolated lysosomes under fed and starved conditions in wild-type and WDR41 KO HeLa cells. Quantification of the levels of C9orf72 (D) and SMCR8 (E) on magnetically isolated lysosomes (mean ± SEM, n = 3, **** p < 0.0001, *** p ≤ 0.001 two-way analysis of variance [ANOVA] with Tukey’s multiple comparisons test).

Article Snippet: GFP-tagged full-length C9orf72 (residues 1–481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1–937, Uniprot identifier Q8TEV9-1) were described previously ( Amick et al. , 2016 ). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand, Université de Strasbourg, Illkirch, France (Addgene plasmid #74159) ( Sellier et al. , 2016 ).

Techniques: Purification, Western Blot, Isolation

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.E17-12-0703

Figure Lengend Snippet: WDR41 localizes to lysosomes in starved cells. (A) Sequencing result from the genomic DNA PCR product from HEK293FT cells that have a 2xHA epitope tag inserted at the endogenous WDR41 locus. The positions of the WDR41 C-terminal sequence, 2xHA epitope tag, and stop codon are indicated. (B) The specificity of the anti-HA immunofluorescence signal in WDR41-2xHA cells is supported by the absence of this signal in parental, non-gene-edited cells. Scale bar, 10 µm. (C) Anti-HA immunoprecipitations from WDR41-2xHA cells followed by immunoblotting for the indicated proteins. WDR41-2xHA retains the ability to interact with C9orf72 and SMCR8. Non-gene-edited HEK293FT cells served as a negative control. (D) Immunofluorescence images showing the localization of WDR41-2xHA (expressed from the endogenous locus) and LAMP1 in fed and starved (1.5-h serum and amino-acid-free) conditions. (E) Immunoblot analysis of WDR41 levels on magnetically-isolated lysosomes from HeLa cells in fed and starved conditions. (F) Quantification of WDR41 immunoblots expressed as a fold increase in the lysosome fraction relative to the input (mean ± SEM, n = 3, unpaired t test, **** p < 0.0001).

Article Snippet: GFP-tagged full-length C9orf72 (residues 1–481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1–937, Uniprot identifier Q8TEV9-1) were described previously ( Amick et al. , 2016 ). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand, Université de Strasbourg, Illkirch, France (Addgene plasmid #74159) ( Sellier et al. , 2016 ).

Techniques: Sequencing, Immunofluorescence, Western Blot, Negative Control, Isolation

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.E17-12-0703

Figure Lengend Snippet: RB1CC1 and autophagy are not required for C9orf72 recruitment to lysosomes. (A) Immunoblot analysis of CRISPR-Cas9-mediated RB1CC1 depletion in the HEK293FT cell line that expresses 2xHA-C9orf72 from the endogenous locus. (B) Parental 2xHA-C9orf72 HEK293FT and RB1CC1-depleted cells were treated with and without concanamycin A (V-ATPase inhibitor) for 2 h and autophagic flux was assessed by immunoblotting. Although wild-type cells accumulate LC3-II when treated with concanamycin A, the RB1CC1-depleted cell line does not. (C) Quantification of the ratio of LC3-II to tubulin (mean ± SEM, n = 3, * p ≤ 0.05, two-way ANOVA with Tukey’s multiple comparisons test). (D) Immunofluorescence analysis of C9orf72 localization in starved wild-type and RB1CC1-depleted cells. Scale bar, 10 µm. (E) Immunofluorescence analysis of C9orf72 and LC3 in starved wild-type and RB1CC1-depleted cells. Starved wild-type cells have distinct C9orf72 and LC3 puncta (predominantly autophagosomes), while RB1CC1-depleted cells maintain C9orf72 puncta but lack LC3 puncta. Scale bar, 10 µm.

Article Snippet: GFP-tagged full-length C9orf72 (residues 1–481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1–937, Uniprot identifier Q8TEV9-1) were described previously ( Amick et al. , 2016 ). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand, Université de Strasbourg, Illkirch, France (Addgene plasmid #74159) ( Sellier et al. , 2016 ).

Techniques: Western Blot, CRISPR, Immunofluorescence

Journal: Molecular Biology of the Cell

Article Title: WDR41 supports lysosomal response to changes in amino acid availability

doi: 10.1091/mbc.E17-12-0703

Figure Lengend Snippet: WDR41-dependent recruitment of C9orf72 to lysosomes is critical for the stimulation of mTORC1 activity by amino acids (A) Immunoblot analysis of phospho-S6 kinase (S6K-T389) levels during starvation (1.5 h) and subsequent amino acid (AA) refeeding (15 min) in WT, WDR41 knockout, and cells stably expressing WDR41-HA in the WDR41 knockout background. (B) Summary of S6 kinase phosphorylation levels normalized to total S6K levels; WT refeed normalized to 1 (mean ± SEM, n = 4, **** p < 0.0001, ** p ≤ 0.01, ANOVA with Sidak’s multiple comparisons test). (C) Schematic diagram of the Lyso-C9orf72-GFP construct. The first 39 amino acids of LAMTOR1 were fused to the N-terminus of C9orf72 followed by a C-terminal GFP tag. (D) Live-cell imaging reveals the successful targeting of Lyso-C9orf72-GFP to lysosomes as illustrated by colocalization with cresyl violet, a fluorescent reporter of lysosome acidity. Scale bar = 10 μm. (E) Immunoblot analysis of phospho-S6K levels during starvation (1.5 h) and subsequent AA refeeding (15 min) in wild-type, WDR41 knockout, and cells stably expressing Lyso-C9orf72-GFP in the WDR41 knockout background. (F) Quantification of S6K phosphorylation levels normalized to total S6K levels (WT refeed normalized to 1; mean ± SEM, n = 4, **** p < 0.0001, ** p ≤ 0.01, ANOVA with Sidak’s multiple comparisons test).

Article Snippet: GFP-tagged full-length C9orf72 (residues 1–481, Uniprot identifier Q96LT7-1) and SMCR8 (residues 1–937, Uniprot identifier Q8TEV9-1) were described previously ( Amick et al. , 2016 ). pCMV6 WDR41-HA was a gift from Nicolas Charlet-Berguerand, Université de Strasbourg, Illkirch, France (Addgene plasmid #74159) ( Sellier et al. , 2016 ).

Techniques: Activity Assay, Western Blot, Knock-Out, Stable Transfection, Expressing, Phospho-proteomics, Construct, Live Cell Imaging